In last few decade, several research attempts in the field of nanoscience and nanotechnology due to its distinctive physicochemical and biological properties. Nanoparticles type like copper oxide nanoparticleshas has most attracted due to their multifarious in biological properties and especially suitable in the area of nanomedicine and biomedical sciences. The metal and metal oxide nanoparticles can be synthesized various approaches such as physical, chemical and biogenic methods. Physical and chemical syntheses of nanoparticles are easily obtained desired structure and high purity of the materials. However, physicochemical techniques are high expensive of regents and equipment consume high energy as well as release of high hazards chemicals to the environmental system, which leads to environmental pollutions and hazards to the human and also aquatic animals. In contrast the biogenic synthesis methods are low cost-effective, reliable, eco-friendly and simple way to synthesis of metal oxide nanoparticles. In this review focused on biogenic mediated (Plant, bacteria and fungi) synthesis of copper oxide nanoparticles for their biomedical application of antibacterial activity.